An Updated Protocol for High Throughput Plant Tissue Sectioning
نویسندگان
چکیده
منابع مشابه
An Updated Protocol for High Throughput Plant Tissue Sectioning
Quantification of the tissue and cellular structure of plant material is essential for the study of a variety of plant sciences applications. Currently, many methods for sectioning plant material are either low throughput or involve free-hand sectioning which requires a significant amount of practice. Here, we present an updated method to provide rapid and high-quality cross sections, primarily...
متن کاملA high-throughput, high-quality plant genomic DNA extraction protocol.
The isolation of high-quality genomic DNA (gDNA) is a crucial technique in plant molecular biology. The quality of gDNA determines the reliability of real-time polymerase chain reaction (PCR) analysis. In this paper, we reported a high-quality gDNA extraction protocol optimized for real-time PCR in a variety of plant species. Performed in a 96-well block, our protocol provides high throughput. ...
متن کاملAn Economical High-Throughput Protocol for Multidimensional Fractionation of Proteins
A sequential protocol of multidimensional fractionation was optimised to enable the comparative profiling of fractions of proteomes from cultured human cells. Differential detergent fractionation was employed as a first step to obtain fractions enriched for cytosolic, membrane/organelle, nuclear, and cytoskeletal proteins. Following buffer exchange using gel-permeation chromatography, cytosolic...
متن کاملa high throughput cdma-based reader collision avoidance protocol for rfid networks (hrcp)
a typical radio frequency identification system (rfid) includes one reader and a number of tags. the reader transmit to commands to the tags with interrogation radio signals, and tags receive the command and then respond with their identification. readers with interrogation signals can read tags’ responses. in rfid systems, when multiple tags respond to one reader simultaneously, tag collision ...
متن کاملSimplified high throughput protocol for northern hybridization.
H.Yang, J.McLeese, M.Weisbart, J.-LDionne, I.Lemaire and R.A.Aubin* Laboratory of Immunopharmacology, Department of Pharmacology, Faculty of Medicine, University of Ottawa; Ottawa, Ontario, 1 Department of Biology, University of Regina, Regina, Saskatchewan and Health and Welfare Canada, Life Sciences Division, Biotechnology Section, Sir F.G.Banting Res. Centre, Tunney's Pasture, Ottawa, Ontari...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Frontiers in Plant Science
سال: 2017
ISSN: 1664-462X
DOI: 10.3389/fpls.2017.01721